Figure 4

Synergistic cytotoxic effects between chaetocin and two epigenetic inhibitors. (a) Cells were incubated in the medium containing different combinations of chaetocin (20 nm), JQ-1 (50 nm) or SAHA (50 μm) for 24 h. Cell viability was studied by MTT assay. (b) Cells were treated with different epigenetic inhibitors and collected by cytospin preparations. Cells were stained with Liu’s stain and differentiated cells were counted under a light microscope. Results represent the mean±s.e. of three independent experiments.