Figure 3 | Blood Cancer Journal

Figure 3

From: Rate of CRL4CRBN substrate Ikaros and Aiolos degradation underlies differential activity of lenalidomide and pomalidomide in multiple myeloma cells by regulation of c-Myc and IRF4

Figure 3

shRNA-mediated knockdown of IKZF1 or IKZF3 inhibits proliferation and induces apoptosis in MM cells. (a) Cell population growth chart of MM1.S (left panel) or U266 (right panel) following DOX-induced shRNA knockdown of either IKZF1 or IKZF3 as compared with Vehicle (Veh) or shLUC control. (b) Apoptosis measured by flow cytometry using Annexin-V (AnnV+; x axis) and ToPro3+ (y axis) staining following either Veh or DOX (Dox, 0.01 μg/ml) treatment of shLUC (left panel), shIKZF1 (middle panel) or shIKZF3 (right panel). MM1.S cells on day 1 (top row), 3 (middle row) and 5 (bottom row). Figures shown are representatives of n=3 experiments. Prominent increases in apoptotic cell numbers compared with shLUC or uninduced Veh control samples are indicated in red as % of total cell numbers representing the sum of the three quadrants enriched for apoptotic cells.

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