Figure 2

Investigation of effects of altered Twist1 expression on cellular growth of AML cells. (a) Effects of Twist1 upregulation in KG1a cells. The KG1a cells were transiently transfected with either a pFLAG-Twist1 plasmid or an empty vector, and the number of surviving cells were counted using trypan blue and recorded at 24, 48, 72 and 144 h. (b) Effects of Twist1 downregulation in THP1 cells. The THP1 cells were transiently transfected with either Twist1-targeted siRNA or scrambled control, and the number of surviving cells were counted using trypan blue and recorded at 24, 48, 72 and 144 h. Each value is the mean±s.d. of three independent experiments.