Figure 2
From: MiR-139-5p is a potent tumor suppressor in adult acute myeloid leukemia
Restoration of miR-139-5p expression delays Hoxa9/Meis1-mediated leukemogenesis. (a) Kaplan–Meier analysis of survival of mice transplanted either with Hoxa9/Meis1/ctrl (n=15) or Hoxa9/Meis1/miR-139-5p (n=12). The graph represents a summary of two independent experiments with two biological Hoxa9/Meis1/replicates. All transplanted mice succumbed to leukemia. Mice transplanted with Hoxa9/Meis1/miR-139 developed leukemia significantly (P=0.0003 log-rank test) slower. (b)Immunophenotypic comparison by flow cytometric analysis of bm cells from deceased mice transplanted with Hoxa9/Meis1/miR-139-5p compared with their respective Hoxa9/Meis1/ctrl transplanted cohort. Donor-derived (CD45.1-positive), GFP-positive cells were stained for c-Kit, CD11b, Gr-1 as well as for a cocktail of lymphoid markers (CD4, CD8a, CD19 and CD45R). (c) Left panel. Changes in morphology analyzed by light-field microscopy of Wright-Giemsa-stained bm cells of deceased leukemic Hoxa9/Meis1/ctrl and Hoxa9/Meis1/miR-139 mice. Bm cells overexpressing Hoxa9/Meis1/miR-139 show a more differentiated morphology based on their nucleus shape and structure. A 100 × magnification of a representative field is shown. Right panel. Blast counts on bm from mice succumbed from Hoxa9/Meis1/miR-ctrl or Hoxa9/Meis1/miR-139 (engraftment of transduced cells between 94 and 99%). Pairwise comparisons were performed using Student’s t-test.
