Figure 1

The TPO/MPL axis is activated in LSK from Gata1^low mice. (a) Representative flow cytometry determinations of cell-surface expression of Mpl in Lin^negSca1⁺Kit⁺ (LSK) cells from wild-type mice, either untreated or treated with TPO, and untreated Gata1^low mice. (b) Quantitative reverse transcription-PCR determinations of the levels of Mpl mRNA expressed by LSK cells from wild-type mice, either untreated or treated with TPO, and from untreated Gata1^low mice (six mice per experimental group). Results are expressed as mean (±s.d.) of relative values when compared to those from untreated wild-type mice. (c) Mean fluorescence intensity (MFI) determinations of the cell-surface expression of MPL by LSK cells in the same groups analyzed in a. Results are expressed as mean (±s.d.) with six mice per experimental point. *P<0.05 with respect to wild-type mice by analysis of variance.