Figure 2

Bone marrow and spleen from Gata1^low mice contain levels greater than normal both of signaling elements downstream to TPO and of those downstream to TGF-β. (a, b) Western blot determinations of the JAK2, STAT5 and pSTAT5 content of bone marrow and spleen from wild-type and Gata1^low mice (each lane). GAPDH was used as loading control. Quantification was obtained by normalizing each band to the corresponding GAPDH level and is presented as mean (±s.d.) with three mice per experimental group. P-values were determined by analysis of variance (ANOVA). (c, d) Western blot determinations of the SMAD2/3, phosphorylated p38 (p-p38), p38 and EZH2 content in the bone marrow and spleen from wild-type and Gata1^low mice (each line a different mouse). GAPDH was used as a loading control. Quantification was obtained by normalizing p-p38 toward the corresponding p38 band, and SMAD2/3, p38 and EZH2 signals against GAPDH. P-values were calculated by ANOVA. EZH2, enhancer of zeste homolog 2.