Table 1 The liver and the plasma from Gata1low mice express greater levels of TPO mRNA and protein than that of piastrinopenic mouse models (bled wild-type mice and Mplnull mice)
Wild type | Wild type, bleeding | Gata1 low | Mpl null | |
---|---|---|---|---|
Blood values | ||||
Hct (%) | 47.8 (±0.3) | 43.1 (±1.1)a | 45.0 (±3.3) | 42.3 (±1.3)a |
Plt/μl (× 106) | 0.9 (±0.03) | 0.7 (±0.03)a | 0.3 (±0.01)† | 0.03 (±0.004)a |
TPO mRNA (2−ΔCt)(× 10−3) | ||||
Liver | 39.3±7 | 41.3±4.3 | 94.2±9.5a | 35.5±10.5 |
Spleen | 1.3±0.1 | 0.5±0.2 | 0.02±0.002a | ND |
Bone marrow | 0.1±0.006 | 3±1a | 0.007±0.001a | 0.01±0.002a |
Bone | 1.9±0.5 | 0.5 | 0.3±0.1a | BD |
Ear | BD | 1.2 | 0.06±0.005 | BD |
Kidney | 6.2±0.001 | 7.6±0.9 | 10.6±1.3 | 8.9±2.1 |
Stomach | 0.7±0.1 | 0.3±0.03a | 4.6±0.9a | 1.0±0.1 |
Intestine | 4.0±0.2 | 8.2±2.0 | 2.9±0.7 | 2.2±0.1a |
TPO protein (pg/ml) | ||||
Plasma | 351±31a | 451±11a | 778±101a | 1287±205 |
Bone marrow | 98±13 | 96±14a | 25±1a | 47±13 |
Spleen | 99±15 | 140±4 | 66±3 | 119±7 |
Liver | 289±28 | 179±6a | 166±10a | 271±74 |
Peritoneum | 93±2 | 88±3 | 45±5a | 97±15 |