Table 1 The liver and the plasma from Gata1low mice express greater levels of TPO mRNA and protein than that of piastrinopenic mouse models (bled wild-type mice and Mplnull mice)

From: The thrombopoietin/MPL axis is activated in the Gata1low mouse model of myelofibrosis and is associated with a defective RPS14 signature

 

Wild type

Wild type, bleeding

Gata1 low

Mpl null

Blood values

 Hct (%)

47.8 (±0.3)

43.1 (±1.1)a

45.0 (±3.3)

42.3 (±1.3)a

 Plt/μl (× 106)

0.9 (±0.03)

0.7 (±0.03)a

0.3 (±0.01)

0.03 (±0.004)a

TPO mRNA (2−ΔCt)(× 10−3)

 Liver

39.3±7

41.3±4.3

94.2±9.5a

35.5±10.5

 Spleen

1.3±0.1

0.5±0.2

0.02±0.002a

ND

 Bone marrow

0.1±0.006

3±1a

0.007±0.001a

0.01±0.002a

 Bone

1.9±0.5

0.5

0.3±0.1a

BD

 Ear

BD

1.2

0.06±0.005

BD

 Kidney

6.2±0.001

7.6±0.9

10.6±1.3

8.9±2.1

 Stomach

0.7±0.1

0.3±0.03a

4.6±0.9a

1.0±0.1

 Intestine

4.0±0.2

8.2±2.0

2.9±0.7

2.2±0.1a

TPO protein (pg/ml)

 Plasma

351±31a

451±11a

778±101a

1287±205

 Bone marrow

98±13

96±14a

25±1a

47±13

 Spleen

99±15

140±4

66±3

119±7

 Liver

289±28

179±6a

166±10a

271±74

 Peritoneum

93±2

88±3

45±5a

97±15

  1. Abbreviations: BD, below detection; Hct, hematocrit; ND, not done; Ptl, platelet counts; TPO, thrombopoietin. Blood values and TPO mRNA and protein levels in tissues from wild-type and Gata1low and Mplnull piastrinopenic mice. Results are presented as mean (±s.d.) of those obtained with three mice per experimental group.
  2. aValue statistically different (P<0.01 by analysis of variance) from those observed in wild-type mice.