Figure 3

PCYT1A was related to mitophagy-dependent necroptosis and disease progression in DLBCL. (a) Protein levels of PCYT1A and MYC in DB and Ramos cells transfected with PCYT1A siRNA or CON siRNA were assessed by western blottig. (b, c) Cell growth (b) and necroptosis (c) were detected in cells transfected with PCYT1A siRNA or treated with mitophagy inhibitor Mdivi-1 (50 μM). (d) Representative immunofluorescence images of LC3A/B (red) and mitochondrial (MTC, green) in DB cells transfected with PCYT1A siRNA1 or CON siRNA. Cells were counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue). (e) PCYT1A gene expression was assessed by quantitative real-time PCR in DLBCL (n=108) and reactive hyperplasia (n=20). (f, g) Tumor MYC expression (f) and serum PC(16:0/22:6) level (g) were calculated according to PCYT1A expression in DLBCL. Data in b, e and g were represented as mean±s.e.m. Assay in b was set up in triplicate.