Figure 3
PpIX accumulation in glioma cells after 5-ALA exposure. (A) PpIX fluorescence spectra tracing at various time points. The peak at 605 nm (arrow) indicates the characteristic PpIX fluorescence spectra. x-axis: wavelength; y-axis: relative intensity. (B) The time-dependent PpIX accumulation curve was drawn according to the results of (A). PpIX fluorescence spectral intensity reached a plateau at ∼6 h in U87 and SNB19. The data are based on three independent experiments, and the error bars show mean (s.d.). (C) Molecular imaging of PpIX was visualised by fluorescence microscopy in G112, SNB19, and U87 cells that were incubated for 6 h with 5-ALA. Upper panels: phase-contrast images; lower panels: PpIX molecular images, insets: higher magnification.
