Figure 4
Efficiency of FECH siRNA silencing in glioma cell lines. (A) FECH siRNA sequence 1 and sequence 2 were transfected into G112 and SNB19 cells. Luciferase was transfected as a negative control, and total RNA was extracted from the cells 72 h later for cDNA synthesis. FECH mRNA expression after transfection was determined by QRT–PCR. (B) Micrographs of FECH protein labeled by green-fluorescent Alexa Fluor 488 dye (Invitrogen, Carlsbad, CA, USA) in G112 and SNB19 glioma cells transfected with siRNA. Green fluorescence intensity significantly decreased in FECH siRNA sequence 1 and sequence 2 compared with control siRNA. Representative micrographs of three independent experiments are shown.
