Figure 5
PpIX fluorescence spectra analysis and molecular images of glioma cells transfected with siRNA. (A) PpIX fluorescence spectra tracing at 6 h after FECH or luciferase siRNA transfection and incubation with 400 μ M 5-ALA. The peak at 605 nm (arrow) indicates the characteristic PpIX fluorescence spectra. x-axis: wavelength; y-axis: relative intensity. (B) Histogram of PpIX relative fluorescence intensity of cells transfected with siRNA after 6-h exposure to 400 μ M 5-ALA. PpIX fluorescence spectral intensity was significantly increased in the cells transfected with FECH siRNA sequence 1 and sequence 2 compared with that in the cells transfected with control siRNA (*P<0.05; **P<0.01; ***P<0.005). Data are expressed as mean (s.d.) of triplicate experiments. (C) PpIX molecular images of glioma cells transfected with FECH siRNA. Molecular images of PpIX were visualized by fluorescence microscopy in G112 and SNB19 glioma cells. After 72-h FECH siRNA transfection, the cells were exposed to 400 μ M 5-ALA for 6 h. Note that strong red fluorescence of glioma cells was observed in cells transfected with FECH siRNA compared with control siRNA. Upper panels: phase-contrast images; lower panels: PpIX molecular images, insets: higher magnification.
