Figure 2
From: Thymidylate synthase as a determinant of pemetrexed sensitivity in non-small cell lung cancer
Effect of TS depletion on pemetrexed sensitivity in TS-overexpressing NSCLC cells. (A) Cells of the indicated lines were transfected or not (−) with nonspecific (Con) or TS siRNAs for 48 h, after which cell lysates were subjected to immunoblot analysis with antibodies to TS and to β-actin. (B) Cells transfected as in A were cultured for 72 h in complete medium containing various concentrations of pemetrexed, after which cell viability was assessed as described in Materials and methods section, and the median inhibitory concentration (IC50) of pemetrexed was determined. Data are means of triplicates from experiments that were repeated on two additional occasions with similar results. *P<0.05 (Student's two-tailed t-test).
