Figure 2

Modulation of protein expression and posttranslational modifications in LECs. LEC monolayers were incubated with 1 μM synthetic 12(S)-HETE and analysed by western blotting after 0.2, 0.5, 1.0, 2.0, and 4.0 h. Equal sample loading was controlled by Ponceau S staining, β-actin (A, B, D), or α-tubulin (C) expression. Co, untreated LECs.