Figure 3 | British Journal of Cancer

Figure 3

From: NF-κB mediates the 12(S)-HETE-induced endothelial to mesenchymal transition of lymphendothelial cells during the intravasation of breast carcinoma cells

Figure 3

Analysis of VE-cadherin expression in LECs. (A) LECs were treated with 1 μ M 12(S)-HETE for 0.2, 0.5, 2, 4, and 8 h. Then, cells were harvested and protein lysates were analysed by western blotting. MCF-7 cells were used as negative control. Equal sample loading was controlled by Ponceau S staining and β-actin analysis. Confocal immunofluorescence images of LECs next to a spheroid (B) and underneath an MCF-7 spheroid (C). LECs were grown on coverslips until confluence when MCF-7 spheroids were transferred on top of LECs and co-incubated for 4 h at 37°C to allow CCID formation. LECs were stained with anti-VE-cadherin antibody (red) and DAPI (blue). (B) Distant to a spheroid, VE-cadherin structures appear well developed, whereas (C) VE-cadherin interactions are disrupted underneath an MCF-7 spheroid. Scale bar: 15 μ M. The colour reproduction of this figure is available at the British Journal of Cancer journal online.

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