Figure 4

Effect of ZEB1 suppression on VE-cadherin regulation by 12(S)-HETE. LECs were transiently transfected with two different siRNAs against ZEB1 (+: siRNA1; +′: siRNA2), or with scrambled siRNA. LECs were subsequently treated with 1 μ M 12(S)-HETE and analysed by western blotting using antibodies against ZEB1 and VE-cadherin. Equal sample loading was controlled by β-actin expression.