Figure 3
Representative sections of dysplastic oral mucosa taken from patient biopsies (DOM) (A–E) and tissue-engineered dysplastic oral mucosa constructs (TEDOM) (F–J). TEDOM models were generated by culturing D20 or Cal27 cells and normal oral fibroblasts on DED at an air-to-liquid interface for 14 days. Sections were stained with H&E (A, F and K) and immunohistochemically stained to show the pattern of expression of Ki67 (B, G and L), AE1/3 (C, H and M), collagen type IV (Col IV; D, I and N) and integrin αvβ6 (E, J and O) and counterstained with haematoxylin. Scale bar indicates 200 μm.
