Figure 4
Representative sections of oral mucosa taken from patient biopsies pathohistologically classified as carcinoma in situ (CIS) (A–E) and a tissue-engineered model of carcinomas in situ (TECIS) (F–J). TECIS models were generated by the addition of a MCTS to the TENOM model and further culture at an air/liquid interface for 14 days. Sections have been stained with H&E (A and F) and immunohistochemically stained to show the pattern of expression of Ki67 (B and G), AE1/3 (C and H), collagen type IV (Coll IV; D and I) and integrin αvβ6 (E and J) and counterstained with haematoxylin. Scale bar indicates 200 μm. In each case, relatively normal tissue is seen to the left and CIS to the right of each section.
