Table 1 Antibodies and antigen retrieval conditions used in this study

From: Development of tissue-engineered models of oral dysplasia and early invasive oral squamous cell carcinoma

Marker

Antibody clone

Dilution/final concentration

Antigen retrieval

Source

Ki67

MIB-1

1 : 50

0.01 M sodium citrate buffer

Dako, Copenhagen, Denmark

AE1/AE3

AE1/AE3

1 : 100

0.01 M sodium citrate buffer

Dako

Collagen IV

COL-94

1 : 500

0.01 M sodium citrate buffer

Sigma, Poole, UK

αvβ6

6.2A1

0.5 μg ml–1

Proteinase K

Generated as described in Weinreb et al (2004)

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