Figure 4
Immunoblotting analysis of endostatin in the lung tissues of WT and MMP-13 KO mice. (A) Representative data of immunoblotting of endostatin in the lung tissues of WT and MMP-13 KO mice. Mice were received intravenous injection of B16BL6 cells and lung tissues obtained on days 0 (Cont), 1, 3 and 7 after the injection were subjected to immunoblotting for endostatin as described in Materials and Methods. β-Actin, a loading control. (B) Time-course changes of endostatin/β-actin ratio on days 0 (Cont), 1, 3 and 7 (n=5 per group). Densitometric analysis was carried out using Image J software. Bars, mean±s.d. *P<0.05.
