Figure 4
Ectopic expression of mir-200c alters expression of signature miRNA, EMT genes, and modulates sensitivity to erlotinib in A549 cells. (A) ZEB1, E-cadherin, mir-34c, mir-301a, and mir-140 levels were determined in A549 cells. The mir-200c precursor was expressed from a plasmid vector, and mir200c-expressing cells were selected with puromycin. Expression of the query genes was evaluated by qPCR in control and transfected cells. The data are the mean fold change, relative to control (A549 parent cells), of two independent experiments each evaluated in triplicate showing standard deviation (s.d.). (B) A549 and A549+mir200c-expressing cells were treated with erlotinib for 72 h in 0.1% serum-containing RPMI. Cell growth was measured by SRB assay in triplicate, averaged, and compared with 0 h controls. Cell growth at 72 h is plotted against erlotinib concentration.
