Figure 5
TGFβ1 treatment has differential effects on EMT in A549 cells compared with ectopic mir-200c expression. (A–C) A549 or A549+mir200c cells were treated for 3 days with TGFβ1 (2 ng ml−1). (A) Cellular morphology is altered in the presence TGFβ1 in both cell lines. (B) Proteins involved in EMT are differentially expressed in the presence of TGFβ1 in A549 and A549+mir200c-expressing cells. ZEB1, E-cadherin, β-catenin, Snail, and pERK1/2 expression was determined by western analysis. α-Tubulin serves as a loading control. (C) Expression of EMT effector mRNA and a panel of signature miRNAs are differentially expressed in A549 and A549+mir200c expressing. Expression of ZEB1, E-cadherin, mir-200c, mir-34c, mir-301a, and mir-140 genes were evaluated by qPCR in control and mir-200c-expressing cells. The data are mean fold change, relative to control (untreated A549 cells), of two independent experiments each evaluated in triplicate. Bars show standard deviation (s.d.).
