Figure 1 | British Journal of Cancer

Figure 1

From: The cyclin-dependent kinase inhibitor p57Kip2 is epigenetically regulated in carboplatin resistance and results in collateral sensitivity to the CDK inhibitor seliciclib in ovarian cancer

Figure 1

(A) Western immunoblotting of PEO1 cell lines without and with 5-aza-2-deoxy-cytidine/trichostatin A treatment (+aza) using SDS–PAGE gels (NOVEX system, Invitrogen) followed by western transfer onto PVDF membranes. Membranes were probed with a primary rabbit polyclonal antibody to p57kip2 sc-8298 (clone H-91) (Santa Cruz, distributors Autogen Bioclear, Calne, UK). Membranes were stripped and reprobed for actin as a loading control. (B) Expression levels of mRNA p57Kip2 in the PEO1 panel of cell lines obtained qPCR with SyBr green and actin as the internal control. Data shown are the mean (and s.d.) for at least three repeat experiments.

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