Figure 2
(A) Dose-response curves for PEO1 (circle), PEO1CisR (triangle) and PEO1CarbR (square) treated with seliciclib (n=>3, with the s.d. shown for each data point (drug concentration used) as error bars). (B) IC50 data obtained from dose-response curves (using MTT) with continuous drug exposure for PEO1 parental (black), PEO1CarbR (vertical shading), PEO1CarbR demethylated (white) and PEO1CisR (horizontal shading) for carboplatin and for seliciclib treatments. All experiments were carried out on at least three separate occasions and data show the IC50 as mean with s.d. as error bars, P-values obtained for comparison of IC50 values for PEO1CarbR cells versus PEO1CarbR-demethylated cells were 0.0055 (*) for carboplatin and 0.016 (**) for seliciclib (paired t-test). For carboplatin the resistance factors obtained for PEO1CarbR was 4.2 and for PEO1CarbR-demethylated was 2.2.
