Figure 3

Cell-cycle analysis for PEO1 cell lines treated with seliciclib (25 μ M) for 48 h. Key: SubG1, white; G1, black; S-phase, grey; G2M, horizontal line. N=3 separate experiments. Cells were stained with PI (see Materials and Methods) and analysed used a Coulter Epics flow cytometer with data analysis using MultiCycle software (Beckman Corp.). The S-phase for treated PEO1CarbR cells versus control gave a P-value of 0.03, indicated as ^*.