Figure 2
Bcl-2 family expression in oesophageal cancer cell lines. (A) Western blot analysis of basal levels of Mcl-1, Bcl-2, Bcl-xL and Bax in KYSE70, KYSE450 and OE21 cells. (B) Mcl-1, Bcl-2, Bcl-xL and Bax expression following treatment with 5-FU (40 μ M – 24 and 48 h) in (i) KYSE70, (ii) KYSE450 and (iii) OE21 cells. β-Actin was a loading control. (C) Basal expression levels of NOXA (hatched boxes) and levels following 5-FU treatment (40 μ M – 48 h, clear boxes) were determined by real-time PCR. β-Actin was used for normalisation. Differential expression was determined using the ΔΔCp formula, KYSE450 and OE21 cell lines were compared with KYSE70 cells, which is given an arbitrary value of 1. Error bars represent s.e.m.
