Figure 3

Effects of 5-FU or HA14-1 alone and in combination on clonogenic recovery of oesophageal cancer cells. (A) KYSE70 cells were treated with 5-FU (10–30 μ M) alone or in combination with HA14-1 (20 μ M) for 24 h. (B) KYSE450 cells were treated with 5-FU (20–40 μ M) alone or in combination with HA14-1 (20 μ M) for 48 h. (C) OE21 cells were treated with 5-FU (10–30 μ M) alone or in combination with HA14-1 (20 μ M) for 24 h. Drug-treated and control cells were counted and 2000 (KYSE70 and OE21 cells) or 1000 (KYSE450 cells) were re-seeded in triplicate wells without drug and allowed to grow for 12–14 days. Colonies were fixed and counted (presented as mean±s.e.m.). Representative wells are shown on the right-hand panels. Asterisks indicate statistical significance determined by t-test (^*P<0.05, ^**P<0.01, ^***P<0.0001).