Figure 1 | British Journal of Cancer

Figure 1

From: PI3K/Akt signalling is required for the attachment and spreading, and growth in vivo of metastatic scirrhous gastric carcinoma

Figure 1

PI3K/Akt pathway is necessary for the attachment and spreading of metastatic gastric cancer cells to type IV collagen. (A) Upper panel: attachment of cells to type IV collagen induced Akt/PKB phosphorylation shown by immunoblotting. Control is shown on the lower panel with polyclonal antibody to AKT (0.5 μg ml−1). Lower panel: pretreatment with LY294002 attenuated Akt/PKB phosphorylation following attachment of cells to type IV collagen. (B) Effect of LY294002 on the attachment and spreading of OCUM-2MD3 cells to type IV collagen. (C) Adhesion of OCUM-2MD3 cells to type IV collagen was performed in the presence of PI3K inhibitor (wortmannin), PKC inhibitor (calphostin C), and MAPK inhibitor (PD98059). OCUM-2MD3 cells were pretreated with/without inhibitors for 30 minutes with wortmannin, 60 min with calphostin C and PD98059, respectively. (D) OCUM-12, MKN-45, and MKN-74 cells were treated with DMSO (conrol) or 20 μ M LY294002. Adhesion to Type IV collagen-coated membranes was assessed after 30 min. *P<0.01 vs control. Values shown are mean±s.d. (n=4).

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