Figure 2 | British Journal of Cancer

Figure 2

From: PI3K/Akt signalling is required for the attachment and spreading, and growth in vivo of metastatic scirrhous gastric carcinoma

Figure 2

(A) Resistant OCUM2MD3 cells overexpressing p85 proteins were screened by immunoblotting with anti-p85alpha antibodies and subcloned by penicillin-cup methods. Clones selected for use in other experiments are shown by a box around the bands on the blot. (B) Expression of cells transfected with empty vector (control), Wp85, and Δp85 by immunoblotting. (C) The activation of Akt of cells with empty vector (control), Δp85 by immunoblotting after attachment to type IV collagen for 30 min. Control is shown on the lower panel with polyclonal antibody to AKT. (D) Adhesiveness of OCUM-2MD3 cells in transfectants with Wp85, Δp85, or the empty vector. (E) The transfectants were investigated for spreading activities. The transfected cells were examined for in vitro adhesion activities as in this figure. *P<0.01 vs control. Values shown are mean±s.d. (n=4).

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