Figure 1
From: PPARgamma inhibits hepatocellular carcinoma metastases in vitro and in mice
Effect of PPARγ on HCC cells motility by wound healing assay. (A) Pronounced expression of PPARγ protein was confirmed by western blot induced in HCC cells (MHCC97L and BEL-7404) treated with rosiglitazone, Ad-PPARγ or rosiglitazone plus Ad-PPARγ. (B) Representative images of the cell motility in MHCC97L and BEL-7404 cells under treatment with Ad-LacZ, Ad-LacZ+rosiglitzaone, Ad-PPARγ, or Ad-PPARγ+rosiglitazone at 0, 24, and 36 h, respectively. (C) Quantification of cell motility was made by measuring the distance travelled by the cells between the two boundaries of the acellular area, and the results of the different treatment group were expressed as a ratio to Ad-LacZ-treated cells (control). The experiment was performed for three times in triplicate. The data are expressed as means±s.d., *P<0.05, **P<0.01, ***P<0.0001, compared with the Ad-lacZ.
