Figure 5
From: The cap-translation inhibitor 4EGI-1 induces apoptosis in multiple myeloma through Noxa induction
‘Mcl-1/Bcl-xL-free’ Noxa is involved in 4EGI-1 induced-apoptosis. (A) ‘Mcl-1-free’ Noxa is detected in Mcl-1 immunoprecipitate supernatant. NCI-H929, LP-1 and OPM-2 cells were cultured with or without 50 μ M 4EGI-1 for 18 h. Cell extracts were immunoprecipitated with an anti-Mcl-1 antibody. Immunoblotting were then performed on both immunoprecipitates and immunoprecipitate supernatant using the indicated antibodies. (B) ‘Mcl-1/Bcl-xL free’ Noxa is detected in Bcl-xL immunoprecipitate supernatant previously depleted of Mcl-1. Bcl-xL was then immunoprecipitated from Mcl-1-depleted lysates, and immunoprecipitates and supernatants were analysed for the presence of Noxa.
