Figure 2
Preparatory experiments using human blood samples containing cancer cells. (A) Phase contrast and fluorescent images of peripheral blood cells containing C33A cervical cancer cells. A total of 100 C33A cells were mixed with 5-ml whole blood samples from healthy volunteers and treated with haemolysis buffer, followed by the infection with 1 × 105 PFU of OBP-401. Green fluorescent protein-expressing cells were analysed 24 h after infection. Green fluorescent protein was mainly expressed in C33A cells (upper lane), but some WBCs expressed GFP (lower lane). (B) The number of the GFP-expressing cells in 5 ml blood with or without 100 C33A cells is shown. The samples were infected with the indicated amount of OBP-401. Bars indicate s.e.
