Figure 6

Phenotypic consequences of overexpression of wild-type LPL or LPL fusion proteins in cervical SCC cells. The top row (A–C) shows the effects of overexpressing wild-type LPL and the PEX5–LPL fusion proteins in SW756 cells, whereas the bottom row (D–F) shows the effects of overexpressing wild-type LPL in C33A cells. (A, D) Expression levels were determined by qRT–PCR, using primers for the C terminus of LPL, which was present in all transgenes. (B, E) Growth curves were generated for stable populations of cells expressing PEX5–LPL, PEX5–LPLvar or LPL, compared with those transfected with empty vector. Error bars indicate the s.e.m. between triplicate wells. There were no significant differences in growth. (C, F) Invasion through basement membrane extract was measured and expressed as the percentage of invading cells relative to control. Error bars indicate the s.e.m. between triplicate assays. Wild-type LPL and the fusion proteins consistently increased the invasiveness of cervical SCC cells.