Figure 3

miR-204 increases apoptotic levels of neuroblastoma cells following treatment with CDDP. Kelly cells were transfected with siRNA targeting BCL2 or siRNA negative control oligonucleotide (siNEG NC), or miR-204 mimics or scrambled negative control (scrambled NC) oligonucleotides. At 48 h post transfection, cells were treated for 24 h with or without 5 μg ml⁻¹ CDDP. Graphed data are mean values ±s.e.m. of at least three biological replicate experiments. P-values were determined using two-sided Student’s t-tests. (A) Caspase 3/7 activation was measured at 72 h post transfection (n=3, with five technical replicates). (B) Mean percentage of annexin V-positive Kelly cells from at least three independent experiments at 72 h post transfection with siBCL2/siNEG NC, or miR-204 mimic/scrambled NC and with/without 5 μg ml⁻¹ CDDP treatment. (C) Representative scatter plots of PI vs Annexin V-FITC staining in one independent experiment with Kelly cells at 72 h.