Figure 2

Mutant frequency (MF) by Spi− assay in bystander lung tissues. Relative fold change of Spi− MF in bystander lung tissues at 24 and 72 h after PBIR compared with control group. The WBIR with 5 Gy but not with scattering dose (6 cGy) increased Spi− MF in lung tissues at 24 h after irradiation. Statistical significance of the difference in MF between control and irradiation groups is marked with asterisks (*P<0.05, Student’s t-test). Error bars for quantification of relative Spi− MF in each group indicate the s.e.m. for three independent animals.