Figure 4
Partial body irradiation (PBIR) induced prostaglandin production and oxidative DNA damage in bystander lung tissues. Compared with control animals, two isoforms of prostaglandin, PGE2 (A) and 8-iso PGF2 (B), were elevated in bystander lung tissues at 24 h after irradiation. (C) The PBIR induced oxidative DNA damage in bystander lung tissues. One representative microscopic field of staining of 8-OHdG, oxidative DNA damage marker in lung tissues of each group at 24 h after irradiation; × 100 magnification. (D) Quantification of 8-OHdG staining intensity in microscopic fields of each group under × 100 magnification. Bar represents 20 μm. Statistical comparison between control and irradiation groups marked with asterisks (*P<0.05, **P<0.01, Student’s t-test). Error bars for prostaglandin level indicate the s.e.m. for three independent mice. Error bars for quantification of 8-OHdG staining in each group indicate the s.e.m. for 20 independent microscopic fields from three different mice.
