Figure 5
Partial body irradiation (PBIR) induced DNA DSBs and apoptosis in non-targeted lung tissues. (A) 53BP1 was induced in bystander lung bronchial epithelial cells. A representative microscopic field of each treatment group under × 40 magnification is shown. (B) Active caspase-3 staining in control and bystander lung tissues under × 40 magnification. Arrows show positive caspase-3 cells. (C) Quantification of the fraction of positive caspase-3 cells assessed by microscopy under × 40 magnification. Bar represents 50 μm. Statistical comparison between control and irradiation groups marked with asterisks (*P<0.05, Student’s t-test). Error bars for quantification of caspase-3 staining in each group indicate the s.e.m. for 20 independent microscopic fields from three different mice.
