Table 1 Primer sequences of GSTM1 and GSTT1 genotype

From: Glutathione S-transferase M1-null genotype as risk factor for SOS in oxaliplatin-treated patients with metastatic colorectal cancer

Primer designation

Sequences

Product length (bp)

GSTM1

First amplification

  

 Forward

5′-GAACTCCCTGAAAAGCTAAAGC-3′

219

 Reverse

5′-GTTGGGCTCAAATATACGGTGG-3′

 

Second amplification

  

 Forward

5′-CAGAGTTTCTGGGGAAGCGG-3′

191

 Reverse

idem first amplification

 

GSTT1

First amplification

  

 Forward

5′-TTCCTTACTGGTCCTCACATCTC-3′

290

 Reverse

5′-AAGACTTGGCAGCCAGCACC-3′

 

Second amplification

  

 Forward

idem first amplification

249

 Reverse

5′-TACAGACTGGGGATGGATGG-3′

 

β -globine

First amplification

  

 Forward

5′-GAAGAGCCAAGGACAGGTAC-3′

268

 Reverse

5′-CAACTTCATCCACGTTCACC-3′

 

Second amplification

  

 Forward

5′-GGCTGGGCATAAAAGTCAGG-3′

162

 Reverse

idem first amplification

 
  1. Abbreviations: bp=base pairs; GSTM=glutathione S-transferase enzyme μ; GSTT=glutathione S-transferase enzyme θ.
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