Figure 3

Pyrosequencing analysis of the NT5E CpG island in WM35 and SKMel173 cell lines. The figure shows representative pyrograms. The shaded C residue is the control for bisulphite conversion: there must be no peak at this residue (non CpG cytosine/guanine), confirming 0% cytosine incorporation. % Methylation at each CpG is indicated.