Figure 5
From: Regulation of tamoxifen sensitivity by a PAK1–EBP1 signalling pathway in breast cancer
Regulation of ErbB2 levels in LTLT-Ca cells by EBP1 overexpression and IPA-3. (A) ErbB2 expression levels were measured by western blot analysis of logarithmically growing vector control, EBP1 wild-type or T261A-transfected LTLT-Ca cells. Expression of the GFP–EBP1 fusion protein and GAPDH was also analysed. (B) LTLT-Ca vector control or wild-type or mutant EBP1-transfected cells were serum-starved overnight in media containing 0.5% FBS with or without 3 μ M IPA-3 as indicated. Cell lysates were collected and analysed by western blotting for ErbB2, GFP–EBP1, pMAPK, MAPK and GAPDH levels as indicated. The numbers below the ErbB2 western blot represent relative densities normalised to GAPDH. The numbers below the pMAPK blot indicate the relative densities of pMAPK normalised to MAPK (p44).
