Figure 3 | British Journal of Cancer

Figure 3

From: A short-term colorectal cancer sphere culture as a relevant tool for human cancer biology investigation

Figure 3

Single-cell adhesion and tissue remodelling mechanisms lead to colosphere formation. (A) Protocol of the co-incubation of dissociated XenoCT320 tissue with CT320 × 6-GFP single cells. Confocal immunofluorescence of derived small (B) and bigger colospheres (C). Visualisation of GFP in green, TOPRO-3 in blue (nuclei), AlexaFluor555-Phalloidin in red (F-actin). Scale bar: 50 μm.

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