Figure 1
From: Rare BRAF mutations in melanoma patients: implications for molecular testing in clinical practice
Pyrograms and Sanger sequencing of rare BRAF V600 mutations.
(A) Wildtype, (B) V600E, (C) V600E2 (GAA variant), (D) V600R, (E) V600K), (F) V600D, and (G) L597S. The height of the signal peaks at the A position before codon 600 and the G signal peak after the codon 600, respectively, as well as the G at the third position of codon 600 discriminate between the six mutant variants. The L597S mutation cannot be detected by pyrosequencing. The arrows indicate the mutated codon in the Sanger sequence. The deceptive letter codes in (B, C, E, G) above the Sanger sequencing panels indicate the need for careful cross-check to define the final mutation.
