Figure 1
Visualisation of disseminated growth of Ewing sarcoma xenografts in mice by WB-MRI. VH-64 cells (2 × 106) were injected into the tail veins of 18 NOD/scid mice, followed by WB-MRI after 3 weeks, and then at weekly intervals. T2 axial sequences were used for detection of lung tumours, and T2 axial, sagittal, and STIR sequences were used for detection of manifestations at all other sites. (A) Numbers of mice developing detectable tumour manifestations at the indicated locations. (B) Total numbers of tumours per individual tumour site. (C) Boxplots of median tumour volumes at first detection at the various locations. The lines indicate the median sizes, and boxes represent the 25th and 75th percentiles. Circles symbolise outliers, asterisks show extreme values. (D) Light microscopy of haematoxylin and eosin (HE)-stained sections (left panel) and immunohistochemistry analysis by huCD99 staining (right panel) of tumours from the indicated organ and tissue sites. Altogether, the Ewing sarcoma origin was confirmed by histology and/or immunohistochemistry in four lungs with multiple lesions, five bone lesions (three in legs, two in pelvis and vertebral column, respectively), and four renal tumours.
