Figure 4
Proliferative responses of lymphocytes. Splenic lymphocytes were harvested at 4 weeks after transplantation and exposed to mitogenic stimulation with ConA in vitro. Proliferation was measured from CFSE dilution. (A) Proliferation rates (n=5–6) of lymphocytes from allografted mice (allo-BMT), and following immunisation with naïve (DCnaive, tumour-inexperienced) and tumour-pulsed DC (DCneuro2a) on days +7 and +14. (B) The impact of tumour lysate on lymphocyte proliferation, expressed as changes normalised against the responses in control medium. (C) Proliferation rates of lymphocytes (n=3–5 in each group) from allografted mice (allo-BMT) and following immunisation with tumour-pulsed DC on day +7 incubated with tumour lysate with and without lipopolysacharide (LPS).
