Figure 2
Effect of different light doses and inhibitor application times on MCF-7 viability. (A, B) Inhibitors added 15 min before PDT and maintained for 24 h. (A) Percentage cell death and (B) Total cell number, subdivided into live cells (white area) and dead cells (shaded area). (C, D) Acute application of inhibitors post-PDT, subsequently maintained to 24 h without inhibitors. (C) Percentage cell death and (D) Total cell number, subdivided into live and dead cells. (E, F) Clonogenic assays. Following PDT and 24 h exposure to glycolysis inhibitors, 1000 live cells per condition were maintained in the absence of inhibitors for 9 days. (E) Dark bars show total colonies formed (>2 cells); white bars show colonies larger than 50 cells. (F) Photo-montage of clonogenic assay from E. Significant differences, compared with ALA-only controls, are denoted by *P<0.05, **P<0.01 for live cells or dead cells, and #P<0.05, ##P<0.01 for total cells.
