Figure 3

Short-term (30–270 min) time-courses. (A) MCF-7 cell death (PI positive cells). (B) Apoptosis (annexin V positive cells). In B, representative scatter plots show the distribution of Annexin V-FITC and PI-stained cells. Cells are classified as ‘live’ (bottom left), ‘dead’ (top left plus top right), and ‘apoptotic’ (bottom right). Bar charts below show quantitative analysis of scatter plots. (C) Reactive oxygen species levels (DCFDA fluorescence). (D) Superoxide levels (DHE fluorescence). Significant differences, compared with ALA-only controls, are denoted by *P<0.05, **P<0.01 for PDT-treated cells, and ^#P<0.05, ^##P<0.01 for dark-maintained cells.