Figure 3
From: Oxidative stress specifically downregulates survivin to promote breast tumour formation
OS induces downregulation of survivin in breast cancer cells and increases their sensitivity. Cells were treated with various concentration of DETA-NONOate (1–4 mM) for various time points and analysed for (A) Survivin expression by western blot analysis (1 mM DETA-NOnoate) (B) Caspase-3 enzymatic activity (C) Survivin and MKP-1 protein expression (3 mM DETA-NONOate) and (D) Survivin protein expression (4 mM DETA-NONOate) by western blot analysis. **P⩽0.01 (compared with 1 mM treatment group). Results are representative of three different experiments.
