Figure 6
From: Oxidative stress specifically downregulates survivin to promote breast tumour formation
Survivin levels remain suppressed in human breast tumours where NOX1 and p67 expression was high. Paraffin-embedded tumour sections from TN and ER+ human breast tumours were analysed by immunohistochemical analysis (left panels, A–C), (A) anti-NOX1 (B) anti-p67 or (C) anti-survivin antibodies. (right panels, A–C) Quantitative image analysis demonstrating relative abundance of specific proteins (D) left panel, 50 μg of pooled (five each) tumour lysates obtained from frozen human tumours were subjected to western blot analysis using NOX1, p67, MKP-1, survivin and β-actin antibodies. Right panel, normalised densitometric ratios. **P⩽0.01 (compared with TN group).
