Figure 2

(A) PC3 cells were transiently co-transfected with MET 3′-UTR and miR-34c or scramble mimics. (B) A schematic picture of the two predicted miR-34c binding site in the 3′-UTR of MET. (C) PC3 cells were transiently co-transfected with MET 3′-UTR and miR-34c mimic or negative control. Mutating either of the miR-34c binding sites abolished the effect. (D) PC3 cells were transiently co-transfected with MET 3′-UTR and antisense LNA miR-34c or scramble antisense control. Mutating either of the miR-34c binding sites did not abolish the effect. Luciferase activity was measured after 24 h and normalised to the co-transfected Renilla.