Table 1 Sequences of primers for cloning MET 3′-UTR
From: The tumour suppressor miR-34c targets MET in prostate cancer cells
Outer forward( Fw) | 5′-GTACTATGTCAAAGCAACAGT-3′ |
Outer reverse (Rv) | 5′-CATCACTTTACTTTAATTGCAT-3′ |
Inner Fw | 5′-CTCTCACTAGTCAAAGCAACAGTCCACACT-3′ |
Inner Rv | 5′-CTCTCAAGCTTGCATGATTTATCAGAACAACT-3′ |
Fw_mut1 | 5′-TGTCCAATGGTTTTTTAAATGCCTGACCTTTAAAAGGCCAT-3′ |
Rv_mut1 | 5′-ATGGCCTTTTAAAGGTCAGGCATTTAAAAAACCATTGGACA-3′ |
Fw_mut2 | 5′-TTAGATACTTGTAAATGCCTATACCTGCAGCTGAACTGAAT-3′ |
Rv_mut2 | 5′-ATTCAGTTCAGCTGCAGGTATAGGCATTTACAAGTATCTAA-3′ |
