Figure 4
Effects of DHT on LPCAT1 mRNA and protein expression and PAF release in prostate cancer cells. Cells were treated with increasing concentration of DHT, and in combination of DHT (10−7 M) and RU486 (10−6 M) or flutamide (10−6 M) for 24 h. The mRNA and protein levels of LPCAT1 in LNCaP cells (A) and PC-3 cells (B) were measured by real-time RT–PCR and western blot analysis, respectively. Representative protein bands were presented on the top of corresponding histogram. Concentrations of PAF in LNCaP (C) and PC-3 (D) cell culture were determined by ELISA. Time course expression of LPCAT1 in LNCaP cells (E) and PC-3 cells (F) treated by DHT-CMO-BSA are also shown. Flu, flutamide; Ru, RU486.Values are presented as mean percent control±s.e.m. (A, B, E, F) or mean±s.e.m. (C, D) for five cultures performed in triplicate. *P<0.05 and **P<0.01 compared with control; #P<0.05 and ##P<0.01 compared with DHT (10−7 mol/l−1).
